Kim Ho-jung began her acting career on stage before making her screen debut in the 1999 film Scent of Love. In 2000, she was cast in Bong Joon-ho’s directorial debut Barking Dogs Never Bite, which won her Best Supporting Actress at the 1st Busan Film Critics Awards. In 2001, she starred in the film Nabi, which earned her the Bronze Leopard for Best Actress citation at the 54th Locarno International Film Festival. The movie also scored her a nomination for Best New Actress at the 22nd Blue Dragon Film Awards. In 2006, she was nominated for Best Supporting Actress at the 5th Korean Film Awards for The Peter Pan Formula. In 2014, she acted alongside Ahn Sung-ki and Kim Gyu-ri in director Im Kwon-taek’s drama film Revivre, which gave her the Best Supporting Actress trophy at the 51st Baeksang Arts Awards. In 2021, she was named Best Actress at the 8th Wildflower Film Awards for A French Woman.

Kim Ho-jung majored in theater and film at Dongguk University.