Go Soo first appeared in a soft drink TV commercial before debuting in the MBC sitcoms My Funky Family and Jump and the KBS2 drama series Ad Madness. In 2000, he was named Best New Actor at the MBC Drama Awards for the series Mothers and Sisters. He then won the New Star Award and the Popularity Award at the 2001 SBS Drama Awards for the romantic melodrama Piano. In 2002, he took home an Excellence Award at the SBS Drama Awards for the series Age of Innocence. He then made his big screen debut in the crime thriller film Some, in which he was nominated for Best New Actor at the 25th Blue Dragon Film Awards and the 42nd Grand Bell Awards. In 2005, Go Soo starred in the SBS revenge drama Green Rose, opposite Lee Da-hae. He won an Excellence Award at the 2005 SBS Drama Awards for his performance in the series. In 2010, he starred in the science fiction action film Haunters, which was a box-office hit. In 2016, he was nominated for a Top Excellence Award at the MBC Drama Awards for the adventure sageuk drama Flower in Prison. In 2018, he was nominated for another Top Excellence Award at the SBS Drama Awards for the medical series Heart Surgeons.


Go Soo began his mandatory military service on March 2, 2006 and was officially discharged on April 25, 2008. He served as a civil service personnel in Gangnam District, Seoul. He married his non-celebrity girlfriend in 2012 after four years of dating. They have three children.