Jung Da-eun debuted with 2Eyes in 2013 with the single album Don’t Mess With Me. That same year, she had a short appearance in the SBS series Heirs. In 2016, she landed her first big screen role in the action-crime film Derailed. In 2018, she became part of Channel A’s Coffee, Do Me a Favor. In 2019, she played the young counterpart of Kim Haneul’s character in the JTBC series The Wind Blows. This was followed by another supporting role in OCN’s Class of Lies. In 2020, she joined the JTBC fantasy drama Mystic Pop-up Bar. In 2021, she was cast in tvN’s action sci-fi series L.U.C.A.: The Beginning. In 2022, she acted alongside Kwak Do-won and Yoon Doo-joon in ENA’s comedy drama There is No Gu Pil-soo.