Jung Eun-ji debuted with the South Korean girl group Apink in April 2011 with the extended play Seven Springs of Apink. Later that year, they were named Best New Female Artist at the 13th Mnet Asian Music Awards held in Singapore. In May 2012, they dropped their first Korean studio album Une Année. In August 2015, they released their debut Japanese full-length album Pink Season. In April 2016, she debuted as a solo artist with the extended play Dream. The album spawned the hit song Hopefully Sky, which she wrote herself. In April 2021, Apink marked their 10th anniversary with the digital single Thank You.

Jung Eun-ji landed her first acting gig in the hit 2012 tvN series Answer Me 1997, where she played the female lead role. Her work in the drama earned her numerous acting citations including Best New Actress at the 49th Baeksang Arts Awards. She and her co-star Seo In-gook also took home several Best OST trophies for the song All For You, which they recorded for the series. In 2013, she appeared in the SBS drama That Winter, the Wind Blows, which won her another Best New Actress citation at the SBS Drama Awards. In 2014, she was named Best Young Actress at the Seoul International Youth Film Festival for her performance in KBS2’s Trot Lovers. In 2019, she made her big screen debut in the horror film 0.0MHz.

Jung Eun-ji has a younger brother named Jung Min-ki.