Jung Jun-ho began his acting career in 1995 and just a year later, he was named Best New Actor at the MBC Drama Awards for Open Your Heart. In 2001, he starred in the box-office hit My Boss, My Hero. In 2002, he acted alongside Kim Jung-eun in another blockbuster movie Marrying the Mafia, which earned him the Popular Star Award at the 23rd Blue Dragon Film Awards. In 2005, he was cast as the villain in the film Another Public Enemy, which was the sequel to 2002’s Public Enemy. In 2007, he played the male lead role in MBC’s The Last Scandal of My Life, which gave him a Top Excellence Award at the MBC Drama Awards. In 2009, he joined the main cast of KBS2’s big-budget spy-action drama IRIS, which scored him an Excellence Award at the KBS Drama Awards. In 2010, he was paired with Kim Nam-joo in the MBC series Queen of Reversals, which won him another Top Excellence Award at the MBC Drama Awards. In 2018, he appeared in JTBC’s mega-hit series SKY Castle.

Jung Jun-ho tied the knot with an MBC announcer in 2011. They have a son and a daughter together.