Jung Yi-seo landed her first big screen role in the 2014 period action film Kundo: Age of the Rampant. In 2017, she had appearances in the movie Real and in the JTBC series Age of Youth 2. In 2019, she bagged a small role in director Bong Joon-ho’s award-winning film Parasite. In 2020, she became part of the tvN fantasy action romance Tale of the Nine Tailed. That same year, she was cast in the films On July 7, Samjin Company English Class, and Josée. In 2021, she played supporting characters in tvN’s Mine and in JTBC’s Snowdrop. In 2022, she joined the hit Netflix zombie series All of Us Are Dead.

Jung Yi-seo attended Dongduk Women’s University.