Lee Jun-hyuk landed his first screen role in the 1991 film Mother, Your Son and has since become active in both TV and film. In 2008, he appeared in director Lee Jun-ik’s film Sunny and in the hit comedy movie Scandal Makers. In 2011, he was cast in his first leading role in the film Animal Town. In 2013, he joined Sung Dong-il and Chinese actress Xu Jiao in the sport-comedy movie Mr. Go. He also bagged supporting roles in the films Tazza: The Hidden Card and The Chronicles of Evil, which were released in 2014 and 2015, respectively. His career received a major boost in 2016 when he played Eunuch Jang in the top-rating KBS2 sageuk Moonlight Drawn by Clouds. His performance in the series won him Best Supporting Actor at the 30th KBS Drama Awards. This was followed by memorable portrayal of supporting characters in tvN’s 100 Days My Prince in 2018 and in SBS’s Stove League in 2019. In 2021, he acted alongside Park Hyung-shik and Han Hyo-joo in the tvN thriller Happiness.

Lee Jun-hyuk and his wife have three children together.