Na In-woo debuted as an actor in the 2013 musical Bachelor’s Vegetable Store. In 2015, he made his official TV debut in the MBC series Shine or Go Crazy. That same year, he was cast in the MBC drama My Mom, which earned him a Best New Actor nomination at the MBC Drama Awards. He continued to build up his acting portfolio in the following years, playing supporting characters in a number of TV series such as MBC’s Golden Pouch in 2016 and JTBC’s Mystic Pop-up Bar in 2020. Later in 2020, he acted alongside Shin Hye-sun and Kim Jung-hyun in the hit tvN fusion sageuk Mr. Queen. His career breakthrough came in 2021 when he replaced Ji-soo as the male lead in the KBS2 sageuk River Where the Moon Rises, which won him Best New Actor at the KBS Drama Awards. He also scored a Best New Actor nomination at the 57th Baeksang Arts Awards for his work in the series. In 2022, he joined the KBS2 reality-variety show 2 Days & 1 Night as a fixed cast member.

Na In-woo attended Dankook University.