Oh Yoo-jin began acting in 2018 with brief appearances in OCN’s God’s Quiz 5 and tvN’s The Cursed. In 2020, she became part of the MBC romance melo Find Me in Your Memory and the tvN webtoon-based series True Beauty. In 2021, she joined OCN’s Dark Hole and tvN’s Bulgasal: Immortal Souls. In 2022, she was cast in her first main role in Wavve’s youth drama Seasons of Blossom.

Oh Yoo-jin’s twin brother named Oh Jae-woong is also an actor.