Park Yoo-na made her acting debut playing a supporting role in the 2015 KBS2 series Sassy Go Go. In 2017, she became part of the first season of tvN’s Forest of Secrets, which took home the Daesang at the 54th Baeksang Arts Awards. She gathered more attention in 2018 for her appearance in the hit JTBC drama My ID Is Gangnam Beauty. That same year, she joined the cast of the top-rating JTBC satirical dramedy SKY Castle. In 2019, she acted alongside IU and Yeo Jin-gu in the tvN dark fantasy drama Hotel del Luna. In 2020, she was cast as one of the main characters in the webtoon-based tvN series True Beauty.

Park Yoo-na majored in practical dance at Hanlim Arts School.