Yoo In-soo debuted as an actor in 2017 through the JTBC series Strong Woman Do Bong-soon. That same year, he landed a minor role in director Jang Hang-joon’s psychological thriller film Forgotten. In 2018, he had appearances in the cable dramas Life, My ID Is Gangnam Beauty, and Mama Fairy and the Woodcutter. In 2019, he joined the cast of the JTBC coming-of-age series A Moment at Eighteen. This was followed by a guest role in Chocolate, which also aired on JTBC from 2019 to 2020. In 2021, he played a supporting role in the KBS2’s At a Distance, Spring Is Green. In 2022, he starred in the Netflix zombie series All of Us Are Dead.