Yoon Ji-hye debuted as an actress in the 1998 supernatural horror flick Whispering Corridors, which gave her a Best Supporting Actress nomination at the 19th Blue Dragon Film Awards. She then appeared in the 2000 coming-of-age movie Plum Blossom, which earned her another nomination for Best Supporting Actress at the 21st Blue Dragon Film Awards. In 2006, she acted alongside Shin Ha-kyun and Kim Min-joon in director Park Chul-hee’s neo-noir action-comedy film No Mercy for the Rude. Her performance in the movie scored her yet another Best Supporting Actress nomination at the 27th Blue Dragon Film Awards. In 2007, she joined the main cast of the MBC series Que Sera Sera. In 2011, she became part of the OCN crime thriller Special Affairs Team TEN and then returned to its sequel aired in 2013. In 2014, she starred in the period action film Kundo: Age of the Rampant, which won her Best Supporting Actress at the inaugural Korean Film Producers Association Awards. In 2018, she played supporting characters in KBS2’s Miracle That We Met and MBC’s Come Here and Hug Me.
Yoon Ji-hye studied theater at the Seoul Institute of the Arts.