Yoon Ji-on made his screen debut in 2016 with small roles in tvN’s Memory and SBS’s Our Gap-soon. He next appeared in the 2017 fantasy action film Along with the Gods: The Two Worlds. In 2019, he played supporting characters in the JTBC series Be Melodramatic and the SBS drama VIP. In 2020, he acted alongside Yoo Seung-ho and Lee Se-young in tvN’s Memorist. That same year, he became part of the Netflix apocalyptic horror series Sweet Home. In 2021, he was cast in the tvN dramas You Are My Spring and Jirisan as well as JTBC’s Monthly Magazine Home. In 2022, he joined the main cast of the MBC fantasy series Tomorrow.

Yoon Ji-on graduated from Kyonggi University’s acting department.