Yoon So-hee made her acting debut in 2013 with a supporting role in the KBS2 sageuk Sword and Flower. That same year, she joined the main cast of tvN’s Let’s Eat. In 2014, she appeared in several TV series such as the tvN rom-com Marriage Not Dating and the SBS sageuk Secret Door. In 2016, she starred opposite L in the eight-episode web drama One More Time, which was later released exclusively on Netflix. In 2017, she became part of MBC’s Ruler–Master of the Mask, which won her Best New Actress at the inaugural The Seoul Awards. In 2018, she played the female lead role in the MBN drama Witch’s Love.

Yoon So-hee was accepted as a chemical and biomolecular engineering student at the Korea Advanced Institute of Science and Technology or KAIST in 2011.